Table 2.

Sequence of the oligonucleotide used for FRET melting

Name	Sequence (5′→ 3′)							Tm Na+ (°C)	Tm K+ (°C)
		L1		L2		L3
f131t	TGGG	T	GGG	TTT	GGG	T	GGGT	56.6	75.1
f161t	TGGG	T	GGG	TTTTTT	GGG	T	GGGT	50.5	68.0
f191t	TGGG	T	GGG	TTTTTTTTT	GGG	T	GGGT	50.1	64.8
f1E1t	TGGG	T	GGG	T15	GGG	T	GGGT	47.0	61.6
f1K1t	TGGG	T	GGG	T21	GGG	T	GGGT	45.1	59.9
f1S1t	TGGG	T	GGG	T30	GGG	T	GGGT	–a	–a
f333t	TGGG	TTT	GGG	TTT	GGG	TTT	GGGT	49.2	55.4
f363t	TGGG	TTT	GGG	TTTTTT	GGG	TTT	GGGT	46.9	49.7
f393t	TGGG	TTT	GGG	TTTTTTTTT	GGG	TTT	GGGT	48.4	48.1
f3E3t	TGGG	TTT	GGG	T15	GGG	TTT	GGGT	46.8	46.3
f3K3t	TGGG	TTT	GGG	T21	GGG	TTT	GGGT	47.3	46.0
f3S3t	TGGG	TTT	GGG	T30	GGG	TTT	GGGT	44.0	–a

Lower case letters (f and t) refer to 5′ FAM and 3′ TAMRA, respectively. Note that the presence of the fluorescent labels and different experimental conditions prevent a direct comparison with T_m values found by UV-absorbance experiments in Table 1.

^aweird / low quality melting profile.