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. 2010 Jul 21;38(21):7689–7697. doi: 10.1093/nar/gkq645

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© The Author(s) 2010. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — dsRNA interferes with miRNA processing. (A) Poly (I:C) inhibited Drosha cleavage of pri-miR-16 and pri-miR-31. Labeled DNA markers were loaded in the right lanes. The amount of poly (dI:dC) or poly (I:C) added to the reactions is indicated at the bottom. Shown are representatives of three independent experiments. (B) Dicer cleavage of pre-miR-16 and three dsRNA substrates, ds30, ds50 and ds120. Dicer cleavage was terminated after 1, 2 or 5 min. RNAs were quantified using a PhosphorImager. Cleavage intermediates (‘I’) and products (‘p’) are indicated. Cleavage percentage is calculated as product and intermediate intensities divided by total (substrate, intermediate and products) intensities. (C) Dicer cleavage of pre-miR-16 and its mimic, ds27 hairpin. The sequences and predicted secondary structures of pre-miR-16 and ds27 hairpin are shown at the top.