Figure 5.

In vitro cleavage of potato mitochondrial tRNA^His precursor transcripts in the presence of recombinant Arabidopsis mitochondrial RNase P, PRORP1. (A) The RNA molecules generated upon incubation of a 365-nt-long potato mitochondrial tRNA^His precursor transcript incubated (+P) or not (–P) with PRORP1 were analyzed on a polyacrylamide gel. Molecular mass markers given in nucleotides are indicated on the left. (B) The circularized 240-nt-long RNA product shown in (A) amplified by RT-PCR using primers P1 and P2 (Figure 1A) was cloned. Clones were sequenced, allowing the analysis of the 5′ extremity. The number of clones corresponding to a cleavage at position G_–1 or at position G₊₁ is given.