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. 2010 Jul 30;38(21):7736–7748. doi: 10.1093/nar/gkq648

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© The Author(s) 2010. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 1. — Scheme showing PR gene promoter structure and the effect of promoter targeted agRNAs on the recruitment of RNAP2 to the transcription start site. (A) The regions targeted by representative agRNA duplexes. Relative to PR-B transcription starting site (TSS), PR-26, PR-11 and PR-9 target −26 to −7, −11 to +8 and −9 to +10 regions, respectively. (B) The non-coding 5′-antisense transcript AT2 starts within the coding region of PR in both T47D and MCF7 cells. ChIP assay evaluates the recruitment of RNAP2 in (C) T47D cells treated by agRNA PR-9; (D) MCF7 cells treated by agRNA PR-11. MM: negative control RNA duplex. Mouse IgG was used as a negative control antibody. The experiments were repeated at least three times. ***P < 0.005 as compared to cells treated with a mismatched RNA (MM). P-values were calculated using the two tailed unpaired Student’s t-test with equal variances. All error bars represent standard deviation.