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. 2010 Jul 30;38(21):7736–7748. doi: 10.1093/nar/gkq648

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© The Author(s) 2010. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Effect of reduced expression of AGO1, AGO2, AGO3, or AGO4 (siRNAs, 25 nM) on silencing by PR-9 (25 nM) in T47D cells. qPCR analysis of PR after a double transfection assay with (A) siAGO1, (B) siAGO2, (C) siAGO3, (D) siAGO4 being added first and PR9 added second (in 72 h). Western analysis of PR protein levels after a double transfection assay with (E) siAGO1, (F) siAGO2, (G) siAGO3, (H) siAGO4 being added first and PR-9 added second (in 72 h). Transfection combinations: MM/PR-9 represents that the first transfection is MM, a control RNA duplex and the second is PR-9, etc. All data were normalized to GAPDH. Error bars represent standard deviations, calculated from three independent experiments. ***P < 0.005 as compared to cells treated with a mismatched RNA (MM). P-values were calculated using the two tailed unpaired Student’s t-test with equal variances.