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. 2010 Jul 25;38(21):7485–7499. doi: 10.1093/nar/gkq652

Figure 11.

Figure 11.

Transcription antitermination. (A) Diagrams of two transcripts of nifH mRNA. The locations of regions where primers (R1 and R2) used in the primer extension assay (B) hybridize are presented by arrows. (B) Expression of nifH and glnB. Total RNA was prepared from the wild-type and gnfK or gnfR mutants after they were grown to the early log phase in the media containing Inline graphic, harvested, resuspended and grown in the Inline graphic-free media. Equal amounts of total RNA for each strain were used to examine the expression of nifH and glnB by primer extension assays.