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. 2010 Jul 25;38(21):7485–7499. doi: 10.1093/nar/gkq652

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© The Author(s) 2010. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — GnfM-binding site. Footprint assays were carried out with GnfM and the upstream region of gnfK (A) or gdhA (B). DNA-binding reactions were conducted with GnfM (lane 1, 0 pmol; lane 2, 0.7 pmol; lane 3, 1.3 pmol; lane 4, 2.5 pmol for gnfK, lane 1, 0 pmol; lane 2, 0.3 pmol; lane 3, 0.7 pmol; lane 4, 1.3 pmol; lane 5, 2.5 pmol for gdhA) and probes (0.2 pmol). G, A, T and C represent sequence ladders. The region protected from or modified by DNase I in the presence of GnfM is indicated by a vertical bar.