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. 2010 Jul 25;38(21):7485–7499. doi: 10.1093/nar/gkq652

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© The Author(s) 2010. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — The two-component GnfL/GnfM system. (A) Purification of GnfL_K and GnfM. The sizes of molecular mass standards are shown in kilodaltons. (B) In vitro phosphorylation assay. GnfL_K and GnfM were tested for phosphotransfer activity in vitro. GnfL_K was incubated at room temperature for 5 min for the autophosphorylation reaction. GnfM was then added and further incubated at room temperature for 5 min for the phosphotransfer reaction. The sizes of molecular mass standards are shown in kilodaltons.