Figure 6.
POLRMT arrest at M1dG and PdG in HSP templates. The 32P-labeled transcripts from (A) HSP Xts templates (HSP Xts X:C and HSP Xts X:T) or (B) HSP Xnts X:C templates containing dG (G), M1dG (M) or PdG (P). Adduct-arrested (X) and run-off (RO) transcripts are indicated. Numbers at the sides indicate DNA marker lengths in lanes L100 and L10. (C) A graph presenting the averages of the percent of transcripts arrested at M1dG and PdG opposite C (white bars) or T (black bars) for at least four independent experiments.