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. 2010 Jul 31;38(21):7830–7844. doi: 10.1093/nar/gkq665

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© The Author(s) 2010. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 6. — DNA binding and ATPase activity of wild-type and GyrB_Δinsert gyrase. (A) EMSAs. Titration of wild-type GyrB:GyrA_ΔCTD is shown at top; the GyrB_Δinsert:GyrA_ΔCTD construct, at bottom. Arrows indicate the positions of free DNA (F) and DNA-protein complex (C). Values above each lane indicate the amount of tetramer in nM. (B) Basal ATPase activity. Wild-type and GyrB_Δinsert gyrase activities are plotted as a function of ATP concentration and fit to a standard Michaelis–Menten kinetic model. (C) DNA-stimulated ATPase activity. Wild-type and GyrB_Δinsert gyrase activities at a constant ATP concentration are plotted as a function of sheared salmon sperm DNA concentration. Data for wild-type gyrase are fit to a single-site binding equation; GyrB_Δinsert gyrase data are fit to a line.