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. 2010 Jul 31;38(21):7830–7844. doi: 10.1093/nar/gkq665

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© The Author(s) 2010. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 9. — Model for the role of the insert in strand passage. An abbreviated schematic of the gyrase catalytic cycle is shown with protein domains colored as indicated. Individual steps of the reaction are depicted as follows: (1) Prior to G-segment binding, the DNA-gate is oriented in a state competent for DNA binding, (2) A G-segment is bound and wrapped, (3) ATP binding facilitates T-segment capture and G-segment cleavage, (4) G-segment opening permits T-segment transport, (5) G-segment closure and T-segment release, and (6) ADP release resets the enzyme for another round of strand passage. The insert (green) is proposed to favor formation of a state competent to bind G-segment DNA (step 1) and to aid T-segment transport (step 4).