Table 1.

Oligonucleotides used in this study

Primer	Sequence	Template
dproFw	CGTGCAGAAGATCTTGCGGATTCAGC	Drosophila melanogaster chromosome 2L; GeneID: 34528,34529
dproRev	GGATCCGCAGAATTCTGGTCTGAAAATAACGCGG	Drosophila melanogaster chromosome 2L; GeneID: 34528,34529
pBS-Fw	ATAGGGCGAATTGGGTACCG	pBlueScript SK+
pBS-Rev	AAAGGGAACAAAAGCTGGAGC	pBlueScript SK+
PUbsd-Fw	TCGGGATGACTTTTGGGTTCTG	Drosophila melanogaster chromosome 2L; GeneID: 34528,34529
PUbsd-R544	AGTGACCAAACTACAGATCCACG	Drosophila melanogaster chromosome 2L; GeneID: 34528,34529
PUbsd-R1057	CCGCTCTAGAACTAGTGGATC	pBlueScript SK+
MEFdut-1168Fw	GCAGGCACAGTGTGATGAAGG	Mus musculus, chromosome 2; GeneID: 110074
MEFdut-1168Rev	GTGGCTTTAACCCACTGGTGAC	Mus musculus, chromosome 2; GeneID: 110074
gdh656_Fw	GACGGTCATCTGATCGTTAACG	Escherichia coli; GeneID: 947679
gdh656_Rev	ACTACGTCATCTTCGGTGTAGC	Escherichia coli; GeneID: 947679

Primers used in the PCR reaction to generate plasmid pBS-dutP: dproFw and dproRev primers. Primers used for the amplification of artificial templates by Taq polymerase in the presence different concentration of dUTP: pBS-Fw, pBS-Rev. Primers used for quantification of uracil in artificial and plasmid DNA samples: PUbsd-Fw, PUbsd-R544 (544-bp amplicon length), PUbsd-R1057 (1057-bp amplicon length). Primers used for quantification of uracil in the genome of mouse embryonic fibroblast cells: MEFdut-1168Fw, MEFdut-1168Rev (1168-bp amplicon length). Primers used for quantification of uracil in E. coli genome: gdh656_Fw, gdh656_Rev (656-bp amplicon length).