Figure 2.

Diagrammatic representation of the 5′ specific amplification of cDNA ends using T-RACE. T-RACE ready cDNA serves as template for an asymmetric PCR reaction using a single gene-specific primer (GSP1) and standard dNTPs (without dUTP). This reaction produces single-stranded target cDNAs that include the adapter sequence comprising standard dNTPs. UDG treatment degrades all non-target cDNAs and adapters containing dUTP. Subsequent PCR using a nested GSP2 with the 5′ T-RACE primer results in the specific amplification of target cDNA ends.