Cultured primary sweat gland epithelial cells under an inverted phase-contrast microscope. a the culture on the first day, The eccrine sweat glands had a clear aspect, and formed an irregular ball in the single-curve tube shape (×200), bar = 50 μm. b The culture on the 12th day, The shape was polygon-like and they grew like cobblestone (×200), bar = 100 μm