Fig. 5.

Immunofluorescence staining of passaged sweat gland epithelial cells on the second day (×200), bar = 50 μm (a, b, c, d), 100 μm (e, f). Cultured cells were positive for CK7, CK18, CK19, CEA, and strongly marked the cytoplasm with a green or red color (a–d), staining with the only secondary antibody (e, f) as negative control without primary antibody. The nucleus was stained blue with DAPI. (a CK7, b CK18, c CK19, d CEA, e IgG-FITC, f IgG-PE)