Table 1.
Specific growth rate (μ) and specific glucose consumption rate (QGlc) in CCO cultures treated with different concentrations of ammonium-chloride or sodium lactate. Specific lactate production rate (QLac) was determined for lactate treated cells only
Ammonium/Lactate concentration (mM) | Ammonium treated cells | Lactate treated cells | ||||
---|---|---|---|---|---|---|
μ (h−1) | QGlc (10−11 mmol h−1 cell−1) | μ (h−1) | QGlc (10−11 mmol h−1 cell−1) | QLac (10−11 mmol h−1 cell−1) | ||
Control | 0.0260 | 6.6 | 0.0235 | 7.2 | 8.3 | |
1 mM | 0.0212 | 6.2 | 0.0218 | 6.9 | 9.2 | |
2.5 mM | 0.0143 | 5.9 | 0.0201 | 7.2 | 8.8 | |
5 mM | 0.0116 | 1.6 | 0.0186 | 7.8 | 9.7* | −13.8** |
7.5 mM | 0.00464 | 0.7 | 0.0139 | 7.3 | −11.5 | |
10 mM | 0.0042 | 0.4 | 0.0112 | 7.8 | −35.4 | |
20 mM | – | – | 0.0074 | 6.3 | −85.7 |
*Value for the first 2 days of culture; **Value beyond day 3 of culture