Table I.

Boltzmann fitting parameters for conductance and fluorescence data from TMRM-labeled BK_Ca channel clones

Clone	Conductance		Fluorescence		n
hSlo-Cless-R207Q-	Vhalf	z	Vhalf	z
	(mV)	(e0)	(mV)	(e0)
R17C	−36 ± 12	1.1 ± 0.20	−72 ± 7.6	0.89 ± 0.11	16
R17C-R20A	−7.7 ± 1.8	0.75 ± 0.06	−52 ± 3.0	0.61 ± 0.02	3
R17C-W203V	29 ± 3.3	1.0 ± 0.02	−74 ± 2.5	0.51 ± 0.02	6
G18C	−25 ± 13	1.2 ± 0.24	−64 ± 3.6	0.75 ± 0.03	18
G18C-R20A	5.6 ± 7.5	0.91 ± 0.11	−32 ± 3.2	0.75 ± 0.02	5
G18C-W203V	18 ± 7.2	0.96 ± 0.05	−88 ± 6	0.68 ± 0.05	8
Q19C	−45 ± 3.5	1.3 ± 0.06	−78 ± 3.3	0.81 ± 0.03	18
Q19C-R20A	−7.2 ± 9.7	0.85 ± 0.06	−59 ± 4.6	0.85 ± 0.03	9
Q19C-W203V	13 ± 9.2	0.93 ± 0.05	−94 ± 11	0.65 ± 0.07	7
R20C	−30 ± 7.5	1.4 ± 0.13	N/A	N/A	13
R20C-W203V	10 ± 12	1.0 ± 0.07	−61 ± 5.0	0.86 ± 0.03	6
Y145C	−26 ± 10	1.2 ± 0.11	−82 ± 5.0	0.74 ± 0.05	14
Y145C-R20A	0 ± 10	1.1 ± 0.11	−43 ± 6.1	0.58 ± 0.03	10
Y145C-W203Va	9.9 ± 2.2	0.86 ± 0.10	−58 ± 9.3	0.57 ± 0.05	8
S202Cb	−46	1.29	−75	1.20
S202C-W203Vab	1.9 ± 4.0	0.98 ± 0.03	−70 ± 2.6	0.66 ± 0.04	18
S202C-R20A-W203V	29 ± 5.3	0.99 ± 0.05	−51 ± 7.2	0.53 ± 0.02	13

Labeling positions: R17C, G18C, Q19C, R20C: S0/N-terminus; Y145C: extracellular to S2; S202C: S3/S4 linker. Errors are ± SEM.

^aPantazis et al., 2010.

^bSavalli et al., 2006.