Figure 6.

Slow recovery from inhibition for the locked-open CFTR. (A–C) Representative traces showing recovery after inhibition of locked-open CFTR channels by CFTRinh-172. (D) The diagram shows the average rate of recovery from inhibition by CFTRinh-172 for WT and E1371S CFTR locked in an open state. The rate of recovery for ATP-gated WT-CFTR was plotted for comparison. The mean rate constants of recovery are: 0.024 ± 0.003 s⁻¹ (n = 17) for ATP-gated WT-CFTR; 0.0042 ± 0.0011 s⁻¹ (n = 10) for WT-CFTR locked in an open state with ATP plus PPi; 0.0026 ± 0.0004 s⁻¹ (n = 18) for E1371S; and 0.0039 ± 0.0005 s⁻¹ (n = 18) for E1371S/ΔR.