Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2010 Dec;136(6):659–671. doi: 10.1085/jgp.201010518

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2010 Kopeikin et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

PMC Copyright notice

Figure 8. — The binding and inhibition of CFTRinh-172 in the closed state of E1371S/ΔR-CFTR channels. The inhibitor was applied for ∼20 s in the absence of ATP and was removed from the perfusion solution for 2 s before the application of ATP. Immediately after the application of ATP, one can observe a transient current increase. However, subsequent spontaneous inactivation of open channels without the inhibitor in the perfusion solution suggests that the binding of the inhibitor does happen in the closed state. Subsequent recovery of the current in the presence of ATP took place very slowly. Fitting the recovery phase with an exponential function generates a rate constant of 0.0035 ± 0.0005 s⁻¹, n = 12, which is not different from the rate constant for inhibition that took place during the open state (Fig. 6).