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. 2010 Nov 29;191(5):1029–1041. doi: 10.1083/jcb.201007008

Figure 2.

Figure 2.

Pcdh19 and Ncad act synergistically. (A and B) Embryos were scored at the 10-somite stage for wild-type (A) or morphant (B) phenotypes. (C) Quantification of the interaction between pcdh19 (pcdh19MO) and ncad (ncadMO) morpholinos. Injection of 0.5 ng pcdh19MO results in 20.6 ± 6.8% (314 embryos from four experiments) morphant embryos, whereas injection of 0.2 ng ncadMO yields 6.2 ± 1.9% (308 embryos from four experiments) morphants. However, 88.4 ± 3.0% of embryos (322 embryos from four experiments) were morphant when coinjected with 0.5 ng pcdh19MO and 0.2 ng ncadMO. (D and E) Lateral view of embryos exhibiting wild-type (D) or morphant (E) phenotype at 28 hpf. (F) Injection of 1 ng pcdh19MO results in 8.8 ± 1.6% morphant embryos (182 embryos from five experiments). Similarly, injection of 0.25 ng ncadMO yields 10.5 ± 2.1% (76 embryos from three experiments) morphant phenotype. In contrast, coinjection of 1 ng pcdh19MO and 0.25 ng ncadMO yields 88.2 ± 3.4% of morphant embryos (76 embryos from three experiments). Error bars represent SEM. Bars, 250 µm.