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. 2010 Nov 29;191(5):1029–1041. doi: 10.1083/jcb.201007008

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© 2010 Biswas et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 8. — Analysis of directional cell movements in wild-type and morphant embryos. (A–C) Subset of cell trajectories from wild-type embryos (A). The colored dots indicate the track origins. (B) A plot of net distance traveled against the sum of individual steps for each cell trajectory. Black line has a slope of 1. Red line is a fit to the data points and has a slope of 0.75. (C) A frequency histogram of cell directedness is shown. (D–F) Subset of cell trajectories derived from pcdh19 morphant embryos (D). (E) A plot of net distance against summed distance for cell trajectories in pcdh19 morphants. A line of best fit through the data points has a slope of 0.32. (F) A frequency histogram of the directedness is shown. (G–I) Subset of cell trajectories derived from ncad morphant embryos (G). (H) A plot of net distance against summed distance for cell trajectories in ncad morphants. A line of best fit through the data points has a slope of 0.28. (I) A frequency histogram of the directedness is shown. (J–L) Subset of cell trajectories derived from morphant embryos injected with low doses of both pcdh19MO and ncadMO (J). (K) A plot of net distance against summed distance for cell trajectories in coinjected morphants. A line of best fit through the data points has a slope of 0.43. (L) A frequency histogram of the directedness is shown. Bar, 15 µm.