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. 2010 Nov 29;191(5):915–922. doi: 10.1083/jcb.201008001

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© 2010 Poulson and Lechler

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Figure 4. — p63 does not control mInsc expression but is required for proper cell division orientation. (A) Western blot analysis of e11.5 and e13.5 epidermis. (B) Western blot of e14.5 wild-type (WT) and p63-null epidermis. (C) Mitotic spindle orientations in e14.5 p63-null epidermis. (D–F) Localization of LGN (green) in anaphase wild-type (D) and p63-null epidermal cells (E and F). (G and H) Localization of Par3 (green) in wild-type (G) and p63-null (H) epidermis. Arrows denote apical accumulation of Par3. (I and J) Localization of β1-integrin (green) in wild-type (I) and p63-null (J) epidermis. (K and L) Localization of laminin (green) in wild-type (K) and p63-null (L) epidermis. Dashed lines indicate the basement membrane. Circles indicate boundaries of mitotic cells. Bars, 10 µm.