Fig. 4.

IFNγ production by iNKT cells in response to α-galcer. PBMC samples were treated with 100 ng/ml α-galcer and IFNγ released detected by ELISPOT assay. Known numbers of PBMC were added per well and the number of iNKT cells responding per 10⁶ PBMC calculated (A). Flow cytometry identified the number of iNKT cell per 10⁶ PBMC therefore the % of iNKT cells able to respond to α-galcer was calculated (B). PBMC responses to PHA was determined as a positive control (C). The data is non-parametric and therefore a Wilcoxon test was performed for statistical analysis, *p < 0.05 and **p < 0.005.