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. Author manuscript; available in PMC: 2010 Dec 1.
Published in final edited form as: Circulation. 2010 Apr 26;121(18):2033–2044. doi: 10.1161/CIRCULATIONAHA.109.895037

Figure 8. TRAF1-silencing in EC and monocytes limits the expression of adhesion molecules and integrines and TRAF1 mRNA expression increases in acute coronary syndromes in humans.

Figure 8

A. Human umbilical endothelial cells and human monocytes were transfected with TRAF1-specific- or scrambled siRNA. Cells were stimulated with TNFα (20ng/ml) for 24h and expression levels of VCAM-1 and ICAM-1 (N=4 each) in endothelial cells, and Integrin beta 1(CD29) and CXCR1 (N=3 each) in human monocytes analyzed by Western blotting. Densitometric results adjusted for GAPDH are presented as mean±SEM on top and as grouped scatter blot, representative blots below.

B. 325 patients undergoing coronary angiography were divided into the three groups: no coronary heart disease (No CHD), stable coronary heart disease (CHD), and acute coronary syndromes (ACS). TRAF1 and GAPDH mRNA was analyzed by quantitative real-time PCR in total blood RNA. Spearman correlation coefficients for continuous variables were used to assess univariate correlations of TRAF1 levels with all variables. Results are presented as mean±standard error computed from the average measurements obtained from each group.