Table 2.

Antibodies

Antibody	Dilution	Incubation
Rabbit anti-bovine cytokeratin(Pan) pab, Zymed Laboratories # 08-0059	Predilute	1 h RT
Rabbit anti-human PHH-3 pab, Millipore # 06-570	1:500	32 min., RT
Rabbit anti-human caspase-3 active pab, R&D systems # AF835	1:900	30 min., RT
Rat anti-mouse CD-31 mab, BD Pharmingen #550274	1:10	ON, 4°C
Rabbit anti-mouse Lyve-1 pab, AbCam # ab14917	1:1,000	30 min., RT
Mouse anti-human D2-40, Dako # M3619	1:25	32 min., RT
Rabbit anti-human VEGF-C pab, Zymed Laboratories # 182255	1:150	ON, 4°C
Rabbit anti-human VEGF-D pab, Santa Cruz # sc-13085	1:100	ON, 4°C
Rat anti-mouse CD-68 mab, AbCam # ab53444a	1:600	30 min., RT
Rabbit anti-rat immunoglobulins, Dako # E0468	1:400	30 min., RT

^aAntigen retrieval 1.3 mg/ml Pepsin 30 min RT

Abbreviations: RT room temperature, ON over night