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. 2010 Nov 2;82(23):9782–9788. doi: 10.1021/ac101975k

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Copyright © 2010 American Chemical Society

This is an open-access article distributed under the ACS AuthorChoice Terms & Conditions. Any use of this article, must conform to the terms of that license which are available at http://pubs.acs.org.

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UDP-pentoses in plants. Panel A shows the SIC for UDP-pentoses (536.00-526.08) in tobacco leaves. The peak labeled * is an unknown UDP-pentose. Panels B and C show the results of testing UDP-xylose epimerase activity in microsomes prepared from mung bean sprouts with either UDP-Xyl (UX in panel B) or UDP-Arap (UAp in panel C) as the substrate. Panels D and E exemplify the results obtained when UDP-arabinopyranose was incubated without (panel D) or with (panel E) recombinant UDP-arabinose mutase. Both enzyme reactions were conducted such that they essentially reached equilibrium.