Figure 4.

Elucidation of vesicle-trafficking pathways that play a role in enucleation. (A) Results of enucleation assays of primary mouse splenic erythroblasts in the presence of various inhibitors of vesicle trafficking. The enucleation efficiencies of erythroblasts in the presence of various concentrations of these drugs are shown as mean ± standard deviation of 3 independent experiments. *P < .05, **P < .01, and ***P < .001. (B) Schematic representation of various pathways of vesicle trafficking. Steps at which endocytic vesicle trafficking is disrupted by each of the inhibitors are depicted. The shaded region highlights the steps of vesicle trafficking that are required for enucleation.