FIGURE 2.

Effect on Kin.46 ribozyme activity of extending or shortening the effector oligonucleotide. For Figures 2, 3, 4, and 6, light gray bars indicate data obtained in the presence of effector oligonucleotides, and dark gray bars indicate data obtained when EO was omitted from the reaction. (A) Sequences for EO₊₅ through EO_-6 are shown 3′-to-5′ below the sequence of Kin.46 J1/4 and PBS strands (structural elements are as detailed in Fig. 1). (B) Log(k_obs) values are plotted relative to the values obtained in the presence of EO₀ or without EO (sample “NO”). (C) Comparison of electrophoretic mobilities of Kin.46 with EOs of varying lengths in a 10% nondenaturing polyacrylamide gel. Labels above the lanes align with bar graphs in B. Identical patterns of relative migration were also seen for all 10 samples run on 8% and 6% native polyacrylamide gels (data not shown).