FIGURE 6.

Effect of internal deletions on split kinase ribozyme activity and allosteric activation. (A) The J1/2 loop was disconnected from the 7 nt substrate strand using hammerhead-kinase tandem ribozyme transcripts to remove 0, 5, 10, or 15 nucleotides, as indicated, or all 20 nucleotides (ΔJ1/2, structure not shown explicitly). The L3 loop was similarly interrupted and deletions were made as indicated. Numbers in the corners of each box indicate the sum of the lengths of the 5′ and 3′ segments of the annealed ribozyme. (B) Activities and allosteric activation of “split” ribozyme constructs compared to wild-type. The light gray bars indicate observed rates in the presence of EO; dark gray bars indicate rates in the absence of EO.