FIGURE 7.

Eyring plots of autokinase activity by Kin.46 mutants with alterations in 3′ tail length. Circles with solid lines, represent original Kin.46 in the presence of EO₀. All other reactions are in the absence of EO. Squares with solid lines indicate ribozyme “-18” (18 nucleotides removed from the 3′ end of the original Kin.46); diamonds with solid lines indicate “-15”; triangles with solid lines indicate “-12”; circles with dashed lines indicate “-9”; squares with dashed lines indicate “-6”; diamonds with dashed lines indicate “-3”; triangles with dashed lines represent original Kin.46 without EO. Error bars reflect standard deviations of three or more measurements at each temperature.