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siRNA knockdown of Oct4 in H1 and H7 cells. (A,C,E,G) Results of qRT-PCR. Relative mRNA levels are shown after normalization against β-tubulin mRNA. mRNA levels in cells transfected with siCon were arbitrarily set as 1. Numbers are mean ±SD (n = 3). (B,D,F,H) Representative Western blot results. Antibodies used are shown on the left. Oct4 protein levels in cells transfected with siCon were arbitrarily set as 100%. (A–F) Results from H1 transfection. (G,H) results from H7 transfection.
