A. Circular dichroism spectra of: 1, echinenone in ethanol; 2, gloeobacter rhodopsin, 4 µM in 0.02 % DDM, 25 mM MOPS, 0.1 NaCl, pH 7.2; 3; after reconstitution with echinenone. B. Comparison of the CD spectra of gloeobacter rhodopsin reconstituted with: 1, echinenone; 2, salinixanthin; 3, salinixanthol; 4, β-carotene. The spectra were scaled to the same amount of rhodopsin (4 µM). Spectrum 2 was adapted from (23).