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. 2010 Aug;1(8):836–846. doi: 10.1177/1947601910381645

Figure 1.

Figure 1.

Induction of apoptosis in human prostate epithelial and cancer cells upon HMG treatment. (A and B) Human prostate epithelial PrEC or cancer LNCaP, PC3, DU145, and HPV7 cells, with or without expressing v-Ha-ras, were treated with HMG (1.0 µM) for 24 hours; DNA fragmentation (A) and annexin V (B) assays were performed. Error bar represents the standard deviation (SD) over 5 independent experiments (n = 5, P < 0.05). (C) The expression of JNK1 in the cells was analyzed by immunoblotting. The even loading of total proteins was normalized by actin. (D) The phosphorylation status of JNK1 in untreated or HMG-treated cells was tested using the antiphosphorylated JNK1 antibody. The even loading of total proteins was normalized by actin. (E) The active status of Akt in the cells was immunoblotted with the antiphosphorylated Akt antibody, and the even loading of total proteins was normalized by Akt.