Figure 3.

Overexpression of JNK1 sensitizes LNCaP and PC3 cells to apoptosis. (A) A JNK1 expression vector was transiently transfected into LNCaP or PC3 cells. After the treatment with HMG, the cytosolic fractions were isolated from the cells and immunoblotted for cytochrome c expression. (B) The cells with or without ectopically expressing JNK1 were treated with HMG and subsequently analyzed by DNA fragmentation assay. Error bar represents the standard deviation (SD) over 5 independent experiments (n = 5, P < 0.05).