Figure 5.

Akt activation is required for HMG-induced apoptosis in prostate cancer cells. (A) In the presence or absence of KP372-1 (1.0 µM), the levels of phosphorylated Akt in HMG-treated LNCaP, PC3, or DU145 cells were examined by immunoblotting. (B) HMG-treated or untreated LNCaP, PC3, or DU145 cells with or without expressing dn-Akt were cultured in the growth medium containing GADD153 antisense, sense oligos, NCA (5.0 µM), or KP372-1. Subsequently, the percentage of the cells with fragmented DNA was measured. Error bar represents the standard deviation (SD) over 5 independent experiments (n = 5, P < 0.05). (C) The prostate cancer cells were transiently infected with wt-Akt or treated with JNK1 inhibitor I. Following the treatment with HMG, the percentages of DNA fragmentation in the cells were measured. Error bar represents the SD over 5 independent experiments (n = 5, P < 0.05).