Table 3.
Gene | Noncancerous tissue | Microsatellite genotype of gastric cancers (%) | Bone marrow | ||||
---|---|---|---|---|---|---|---|
H. pylori negative (n = 100) |
H. pylori positive (n = 100) |
LOH-B (n = 13) |
LOH-L (n = 29) |
LOH-H (n = 24) |
MSI (n = 4) |
(n = 18) (%) |
|
Overmethylation frequency | |||||||
CDH1 | 21 | 77* | 0 (0)* | 7 (24) | 3 (13) | 0 (0) | 0 (0) |
ARRDC4 | 20 | 62* | 3 (23) | 8 (28) | 1 (4)* | 1 (25) | 0 (0) |
PPARG | 40 | 82* | 5 (38) | 7 (24) | 6 (25) | 1 (25) | 0 (0) |
CDKN2A | 46 | 90* | 9 (69) | 16 (55) | 9 (38) | 2 (50) | 4 (22) |
TRAPPC2L | 6 | 21* | 6 (46)* | 8 (28)* | 1 (4) | 1 (25) | 0 (0) |
DUSP6 | 12 | 36* | 3 (23) | 3 (10) | 1 (4) | 2 (50) | 0 (0) |
MLH1 | 19 | 37* | 4 (31) | 17 (59)* | 11 (46) | 3 (75) | 0 (0) |
RUNX3 | 42 | 85* | 10 (77) | 26 (90)* | 19 (79)* | 4 (100) | 18 (100) |
PGA5 | 12 | 13 | 5 (38) | 8 (28) | 1 (4) | 0 (0) | 0 (0) |
PGC | 4 | 10 | 4 (31) | 4 (14) | 5 (21) | 1 (25) | 18 (100) |
TFF1 | 37 | 52 | 5 (38) | 3 (10)* | 3 (13)* | 0 (0) | 18 (100) |
TFF2 | 4 | 3 | 4 (31) | 4 (14) | 1 (4) | 0 (0) | 2 (11) |
MSLN | 12 | 11 | 8 (62)* | 16 (55)* | 6 (25) | 1 (25) | 18 (100) |
KRT6A | 18 | 16 | 4 (31) | 16 (55)* | 4 (17) | 3 (75) | 3 (17) |
Undermethylation frequency | |||||||
CDH1 | 0 | 0 | 0 (0) | 0 (0) | 0 (0) | 0 (0) | 0 (0) |
ARRDC4 | 0 | 0 | 0 (0) | 0 (0) | 0 (0) | 0 (0) | 0 (0) |
PPARG | 0 | 0 | 0 (0) | 0 (0) | 0 (0) | 0 (0) | 0 (0) |
CDKN2A | 0 | 0 | 0 (0) | 0 (0) | 0 (0) | 0 (0) | 0 (0) |
TRAPPC2L | 13 | 11 | 1 (8) | 2 (7) | 7 (29) | 1 (25) | 16 (89) |
DUSP6 | 26 | 25 | 3 (23) | 13 (45) | 12 (50) | 2 (50) | 18 (100) |
MLH1 | 20 | 18 | 7 (54) | 6 (21) | 9 (38) | 1 (25) | 17 (94) |
RUNX3 | 5 | 1 | 0 (0) | 1 (3) | 2 (8) | 0 (0) | 0 (0) |
PGA5 | 10 | 14 | 0 (0) | 3 (10) | 5 (21) | 0 (0) | 0 (0) |
PGC | 29 | 18 | 4 (31) | 8 (28) | 5 (21) | 2 (50) | 0 (0) |
TFF1 | 11 | 1* | 7 (54) | 19 (66)* | 17 (71)* | 3 (75) | 0 (0) |
TFF2 | 36 | 26 | 4 (31) | 11 (38) | 8 (33) | 3 (75) | 13 (72) |
MSLN | 0 | 0 | 0 (0) | 1 (3) | 1 (4) | 2 (50) | 0 (0) |
KRT6A | 17 | 16 | 3 (23) | 3 (10) | 3 (13) | 1 (25) | 5 (28) |
The gastric cancers were categorized into baseline-level LOH (LOH-B), low-level LOH (LOH-L), high-level LOH (LOH-H) and microsatellite instability (MSI). The details are described in the material and methods section.
*H. pylori-positive gastric mucosa and gastric cancers were compared with H. pylori-negative gastric mucosa. Significant differences were determined using Fisher's exact test (P < 0.01).