Figure 1.
DT3 protects mouse bone marrow hematopoietic cells after γ-irradiation. (A) Protective effect of DT3 on the survival of irradiated CD2F1 mice (n =16). DT3 or vehicle sc 24 h before TBI (8.75 Gy at a dose rate of 0.6 Gy/min). The difference in 30-day survival between vehicle-injected and DT3-injected groups was statistically significant (P<0.01). (B) Total live cell counts of mouse bone marrow myeloid cells from pooled femur and humerus samples from vehicle control (N=6) and DT3-treated (−24 h or +6 h irradiation) mice (N=6) 1, 8, and 13 days post-irradiation. Total live cell counts of mouse bone marrow myeloid cells 8 days are shown in the upper panel (y axis: 104/mouse). Means±SD. **P<0.01, DT3-treated versus vehicle-treated mice. (C) HE staining of bone marrow from DT3-treated and control mice 8 days post-irradiation (8.75 Gy): longitudinal sections of entire sterna from representative mice in different groups at 20X magnification are shown in the upper panels (panels 1, 2, and 3). Sections in the bottom panels (4, 5, and 6) are higher magnifications of the selected areas of each corresponding upper panel indicated by rectangles (200X). Megakaryocyte foci are identified by arrows.