Figure 3.

Effects of DT3 on Erk, activation of mTOR and S6, and 4EBP-1 phosphorylation in CD34⁺ cells. (A) Western blot shows total Erk1/2 protein and p-Erk1/2 expression in DT3- or vehicle-treated CD34⁺ cells 4 h after 2 and 4 Gy-irradiation. β-actin served as the internal loading control. (B) Immunofluorescence staining shows p-Erk1/2 (green) expression in unirradiated CD34⁺ cells 24 h after DT3 addition. DAPI (blue) defines cell nuclei. (C) m-TOR, S6, and AKT expression and phosphorylation in vehicle control- or DT3-treated CD34⁺ cells were determined by western blot 4 h after 2 Gy irradiation. (D) Western blot: 4EBP-1 was detectable in all samples, and expression and phosphorylation were significantly increased in DT3-treated samples. Representative immunoblots and statistical data from three experiments are shown. Means ± SD. *P<0.05, **P<0.01. 0 Gy versus 2Gy or DT3-treated versus vehicle-treated.