Figure 2.

Histone deacetylases are required for chromatin condensation of cultured mouse fetal liver erythroblasts. (A) Confocal microscopy analysis of TSA-treated mouse fetal liver erythroblasts. TER119-negative mouse fetal liver erythroblasts were cultured as in Figure 1. After 30 h, the indicated amount of TSA was added. Cells were harvested after 42 h and analyzed by confocal microscopy. Z-projections of DNA (blue) are shown. The scale bar represents 5 μm (B) Quantification of the estimated nuclear volume of the cells shown in A and VPA-treated cells. *P<0.001, **P<0.0001. (C) The same cells as in A treated with 10 nM of TSA after 30 h in culture were harvested after 48 h and stained with Alexa Fluor 488–phalloidin. The scale bar represents 8 μm.