Figure 5.

Depletion of HDAC2 blocks chromatin condensation and contractive actin ring (CAR) formation of cultured mouse fetal liver erythroblasts. (A) Quantification of the estimated nuclear volume of 2-day cultured mouse fetal liver erythroblasts infected with lentiviruses expressing HDAC2 shRNA. (B) Alexa Fluor 488–phalloidin staining the same cells as in A. The scale bar represents 5 μm. (C). Quantification from B of cells containing a CAR. Three independent experiments were performed with roughly 200 cells counted in randomly picked areas.