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. 2010 Aug 26;95(12):2022–2030. doi: 10.3324/haematol.2010.025924

Figure 1.

Figure 1.

AML-DC express a functionally active IDO1 protein. (A) Real-time quantitative polymerase chain reaction of IDO1 mRNA in AML blasts and after differentiation into DC (immature AML-DC, mature AML-DC). Results represent the mean ± SD of eight different experiments. (B) Western blot analysis of IDO1 protein. Lanes are as following: 1) IDO1- AML blasts; 2) immature AML-DC; 3) mature AML-DC. The results are representative of six different experiments. (C) Real-time quantitative polymerase chain reaction of IDO1 mRNA in IDO1+ and IDO1− AML samples before and after differentiation to DC. The results are the mean ± SD of six different experiments. (D) L-kynurenine production of AML blasts and AML-DC at different stages of maturation. The results are the mean ± SD of six different experiments. AML blasts include IDO1−and IDO1+ AML samples. (E) Allogeneic T-cell proliferation induced by AML blasts and AML-DC in the presence and absence of 1-methyl tryptophan (1-MT) (1000 μM). The results are the mean ± SD of six different experiments.