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. 2010 Aug 26;95(12):2022–2030. doi: 10.3324/haematol.2010.025924

Figure 5.

Figure 5.

Treg induced by IDO1-expressing AML-DC inhibit the WT1-specific CD8 T-cell response. AML-DC were pulsed or not with 10 mg/mL HLA-A0201-restricted WT1 126–134 for 4 h in complete medium. Then, pulsed or not pulsed AML-DC were cultured with 105 autologous CD3+ T cells at a ratio of 10:1 (T:DC) with 10% autologous serum supplemented with IL-15, IL-7 and IL-2. AML-DC-induced Treg were added or not to cell cultures at a 1:1 ratio with stimulating AML-DC. (A) CD8+ T cells were tested for WT1-specific intracellular interferon-γ production after 24 h of culture. (B) CD8+ T cells were tested for the frequency of WT1-specific CD8+ T cells, by using recombinant human MHC pentamers, after 7 days of culture. Results are representative of three independent experiments.