Figure 6.

T_reg induced by IDO1-expressing AML-DC inhibit the full maturation of normal monocyte-derived DC. Normal immature monocyte-derived DC were matured in the presence of an optimal concentration of lipopolysaccharide (1 mg/mL) and CD4⁺CD25⁺ T_regs, obtained after culture with AML-DC and highly purified. As control samples, purified CD4⁺CD25⁻ T cells, obtained from the same culture, were used. Surface expression of maturation-related markers was tested on DC and expressed as the percentage of positive cells. Results are representative of three independent experiments.