Pilot screening results from the Prestwick FDA-approved drug library. (A) Pilot screen scatter for the 1120 compound Prestwick library. Response in this assay is calculated as a percent-effect as compared with the positive control. A cursory hit threshold is drawn at <32% effect, representing the μ + 3σ (B) Dose response confirmation for the active compound niclosamide (inset) showing a 2.4 μM EC50 and correlating western blot (left lane = DMSO, right lane = positive control) normalized to tubulin. Increased GFP expression correlates with increased peroxisomal specific antigen PMP-70 (↑33%).
Abbrevation: U, arbitrary fluorescent units.