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. Author manuscript; available in PMC: 2011 Nov 19.
Published in final edited form as: FEBS Lett. 2010 Oct 28;584(22):4570–4574. doi: 10.1016/j.febslet.2010.10.052

Figure 4.

Figure 4

Palmitoylation protects RGS4 from oxidation-sensitive degradation. HEK293 cells were transfected with RGS4C2S, RGS4 or RGS4 and DHHC3, cultured under hypoxia for 24 hrs and then transferred to normoxia in the presence of cycloheximide (100 μM). Oxidation-induced degradation of pre-accumulated RGS4 or its mutant was examined over time by western blot assays. (A). Representative western blot. MG132 blocked RGS4 degradation. (B). Time-course of RGS4 degradation obtained from three independent experiments. RGS4 protein was quantified by densitometry and initial optical density units in each group were set as 1. Points, mean; bars, SE.