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In untreated cells, the nuclei of the cells appear green (hoescht) and iNOS expression was undetected (DMEM). iNOS (DyLight™ 488, magenta), was expressed in the cell cytoplasm of stimulated cells. Pretreatment with aminoguanidine (AG) or 17-O-acetylacuminolide (AA) was able to inhibit iNOS expression in cells stimulated with lipopolysaccharide and interferon gamma (LPS+IFNγ).
