Figure 9. Effect of AA on TNF-stimulated L929 cells.

NF-κB (Dylight™488, light blue); was sequestered in the cytoplasm of cells treated with media only (DMEM), the nuclei of the cells appear red (Hoescht). Upon TNF stimulation (TNF), NF-κB translocated to the nucleus. Pretreatment with curcumin (curc.), or 17-O-acetylacuminolide (AA) was able to prevent NF-κB translocation in the presence of TNF. (AA IC₅₀ = 10.9 µgmL⁻¹).