Figure 11. The inhibitory effect of 17-O-acetylacuminolide (AA) on the increase of nuclear NF-κB intensity.

Cells were pretreated with the indicated doses of AA or with 0.1 mM of the NF-κB inhibitor Curcumin (Curc). L929 cells were stimulated with TNF (1 ngmL⁻¹), whereas RAW264.7 cells were stimulated with LPS (10 ngml⁻¹) to cause NF-κB translocation to the nucleus, or were left untreated (DMEM). Data is the average of three independent experiments (±SD), and was analyzed using one way ANOVA with Tukey's post test. The effect was considered significant when groups were compared to TNF (#) or LPS (*) treated L929 and RAW264.7 cells, respectively. (# p<0.05, ** p<0.01, ###, *** p<0.001).