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. 2010 Dec 1;5(12):e15105. doi: 10.1371/journal.pone.0015105

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Achoui et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Human, recombinant IKKβ (5 ng) was incubated in the presence of increasing concentrations of AA as outlined in the manufacturer's detailed protocol. AA dose-dependently inhibited IKKβ activity with an EC₅₀ of 5.2 µgmL⁻¹. Results are average of three independent experiments ±SD. Data was analyzed using one-way ANOVA, with Tukey's post hoc test (* p<0.05, ** p<0.01, *** p<0.001).