Table 1. Comparing protein concentrations and gene expression of 5 pro-inflammatory cytokines in untreated cells and stimulated cells.
| Sample | Protein Conc. Supernatant (pgmL−1) (±STDEV) | P value | Protein Conc. Lysate (pgmL−1) (±STDEV) | P value | Normalized mRNA (fold-change) (±STDEV) | P value | |
| TNF | Stim. | 2510.71 (±708.32) | ** | 122.31 (±41.03) | ** | 41.54 (±13.64) | ** |
| DMEM | 17.17 (±3.88) | 2.01 (±0.32) | 2.14 (±0.57) | ||||
| IL-1β | Stim. | 212.06 (±28.49) | *** | 21.28 (±6.22) | ** | 39.80 (±14.57) | ** |
| DMEM | 6.68 (±4.73) | 3.08 (±0.00) | 0.15 (±0.08) | ||||
| IL-6 | Stim. | 60.64 (±4.37) | *** | 0.75 (±0.18) | * | 0.61 (±0.39) | ns |
| DMEM | 1.22 (±0.00) | 0.06 (±0.00) | 0.25 (±0.19) | ||||
| KC/CXCL-1 | Stim. | 21.81 (±3.17) | *** | 1.43 (±0.43) | ** | 46.82 (±26.00) | ns |
| DMEM | 1.22 (±0.00) | 0.25 (±0.00) | 28.07 (±22.99) | ||||
| GMCSF/CSF-2 | Stim. | 38.46 (±4.49) | *** | 5.16 (±0.39) | Ns | 25.07 (±13.43) | ns |
| DMEM | 1.42 (±0.35) | 4.41 (±0.99) | 13.80 (±11.14) |
Cells were either left untreated in medium (DMEM), or were stimulated with 1 µgmL−1 LPS (Stim.) for four hours (stimulated). The table provides a comparison in protein concentrations (Protein Conc.) in the cell supernatant and lysates, as well as differences in gene expression. Student's t-test was used to calculate the statistical significance between the two groups (ns: not significant, * p<0.05, ** p<0.01, *** p<0.001).